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Safranine O Safranine o or basic red 2 is used as a counter stain in some staining protocols, coloring all cell nuclei red. This is the classic counter stain in a Gram stain. It can also be used for the detection of cartilage, mucin and mast cell granules. Features:
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CAS No.
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477-73-6
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C.I.No.
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50240
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Chemical Formula
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C20H19ClN4
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Molecular Weight
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350.85
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Dye Content
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~95%
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Absorption Max (EtOH 50%)
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530 - 534 nm
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Absorptivity (1%,1cm)
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> 1350 (on dry basis)
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Loss On Drying (110° c)
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8% max
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