Safranin O

Safranin O Specification

Grade Standard
Laboratory Grade

Shelf Life
3 Years

Ph Level
6.5 7.5 (1% solution in water)

Packaging Type
Plastic Bottle / Glass Bottle

Chemical Name
Safranin O

Temperature Range
Store at 15C 30C

Concentration
Certified Strength, Typically 1% (w/v) Solution

Equipment Type
Chemical Reagent

About Safranin O

Safranin O Stain

Safranin O or basic red 2, Safranin o stain is used as a counter stain in some staining protocols, coloring all cell nuclei red. This is the classic counter stain in a Gram stain. It can also be used for the detection of cartilage, mucin and mast cell granules.

Certified Laboratory Grade Quality

Safranin O is manufactured to meet rigorous laboratory-grade standards, ensuring precise performance in scientific applications. Each batch is tested for purity, typical concentration, and pH stability, making it suitable for both educational and professional research environments. Our commitment to quality guarantees results you can trust every time.

Optimal Packaging and Longevity

Choose between plastic and glass bottles based on your laboratorys requirements. All containers are designed to maintain reagent integrity and prevent contamination. With a generous three-year shelf life and recommended storage temperature of 15C to 30C, Safranin O remains stable and effective throughout its usage.

FAQs of Safranin O:

Q: How should I prepare and store Safranin O for laboratory use?

A: To use Safranin O, simply dilute as required or use the provided 1% (w/v) solution. Store it at a controlled room temperature between 15C and 30C, away from direct sunlight, to ensure maximum stability and a shelf life of three years.

Q: What applications is Safranin O most commonly used for?

A: Safranin O is widely utilized as a biological stain in microbiology, botany, and histology procedures. Its main benefit lies in providing clear, contrasting colors, aiding in the visualization of cell structures and tissue differentiation under a microscope.

Q: When is Safranin O the preferred staining reagent in laboratory protocols?

A: Safranin O is preferred during Gram staining as a counterstain and in cytological studies for highlighting nuclei and other cellular components. Its consistent performance makes it ideal when clarity and differentiation are critical.

Q: Where can I purchase Safranin O of reliable laboratory grade quality?

A: Safranin O can be sourced directly from leading exporters, manufacturers, and suppliers in India, known for their adherence to stringent laboratory standards. Ensure you purchase from reputable providers for authentic and certified quality.

Q: What process is recommended for using Safranin O in staining applications?

A: To use Safranin O, apply the 1% (w/v) solution to prepared samples following standard staining protocols. Allow adequate contact time, then rinse excess stain and observe the specimen. Always consult your laboratorys safety data sheet before handling.

Q: What are the benefits of using certified Safranin O in research and diagnostics?

A: Certified Safranin O ensures accurate staining results, reproducibility, and safe handling. Its stable pH and concentration provide clear visualization in various staining methods, benefitting both educational and diagnostic laboratory work.

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